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American Society of Microbiology ABMM Sample Questions:

1. During an investigation of a foodborne outbreak linked to a church supper, 80 people ate the suspected chicken dish, and 32 subsequently developed gastroenteritis. Among 50 people who did not eat the chicken dish, 5 developed similar symptoms. What is the attack rate among those who ate the chicken dish?

A) 40%
B) 10%
C) 64%
D) 5%

2. A clinical microbiology laboratory is preparing to validate a new molecular assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA). To assess the positive predictive value (PPV) of the assay, they need to know:

A) The number of true negative results divided by the total number of negative test results.
B) The number of true positive results divided by the total number of true positive and false negative results.
C) The number of true negative results divided by the total number of true negative and false positive results.
D) The number of true positive results divided by the total number of positive test results.

3. A microbiology laboratory is performing antimicrobial susceptibility testing using Etest strips. The MIC value is determined by:

A) The presence or absence of growth at a single critical concentration.
B) The lowest concentration at which the elliptical zone of inhibition intersects the strip.
C) The highest concentration that allows visible bacterial growth within the zone of inhibition.
D) The diameter of the zone of inhibition around the strip.

4. A centrifuge rotor containing capped tubes of sputum concentrate for AFB culture breaks during operation inside a Class II Biological Safety Cabinet (BSC). What is the most appropriate immediate action for the technologist?

A) Open the centrifuge within the BSC, discard broken glass in a regular sharps container, and clean the spill with 10% bleach.
B) Carefully remove any intact tubes, wipe them with 70% alcohol, and place them in a new rotor to continue processing.
C) Immediately turn off the BSC, open the sash fully, and spray disinfectant widely.
D) Keep the BSC running, notify the supervisor, evacuate the immediate area, and post warning signs.
Allow aerosols to settle (e.g., 30 min) before initiating decontamination with a tuberculocidal disinfectant.

5. A patient develops a localized skin infection characterized by a painless, progressive ulcer with raised, indurated margins. Microscopic examination of tissue scrapings reveals amastigotes within macrophages. The patient reports recent travel to Central or South America. The MOST likely diagnosis is cutaneous:

A) Sporotrichosis
B) Leishmaniasis
C) Myiasis
D) Chromoblastomycosis

Solutions: