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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Clinical Chemistry20-25%- Carbohydrates, lipids, proteins, and enzymes
- Endocrinology and therapeutic drug monitoring
- Toxicology and liver/kidney function tests
- Electrolytes, acid-base balance, and blood gases
Topic 2: Immunology and Serology5-10%- Immunologic testing methodologies
- Immune system principles and components
- Autoimmune and immunodeficiency disorders
- Infectious disease serology
Topic 3: Urinalysis and Other Body Fluids5-10%- Urine formation and composition
- Semen and amniotic fluid testing
- Physical, chemical, and microscopic analysis
- Cerebrospinal, serous, and synovial fluids
Topic 4: Blood Banking (Immunohematology)15-20%- Blood group systems and antigens
- Compatibility testing
- Antibody detection and identification
- Blood components and storage
- Transfusion reactions and adverse events
Topic 5: Hematology20-25%- Hemostasis and coagulation testing
- Red blood cell physiology and disorders
- White blood cell morphology and diseases
- Routine and automated hematology procedures
Topic 6: Laboratory Operations5-10%- Pre-analytical, analytical, and post-analytical processes
- Quality control and quality assurance
- Laboratory safety and regulatory compliance
- Laboratory mathematics and instrumentation
Topic 7: Microbiology15-20%- Specimen collection and culture techniques
- Identification of pathogenic microorganisms
- Mycology, parasitology, and virology
- Bacteriology and antimicrobial susceptibility

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Malbranchia species share the production of alternate staining arthroconidia as a common feature with the mold form of Coccidioides immitis.
Both Geotrichum species and Trichosporon species produce rectangular-shaped arthroconidia; however, they are regularly rather than alternately staining. Additionally, the arthroconidia of Geotrichum may produce germ tubes from one corner and the arthroconidia of Trichosporon species may produce blastoconidia from adjacent corners, features not shared by either Malbranchia species or Coccidioides immitis.
The hyphae of Microsporum canis, as seen in direct KOH mounts of skin scales, may break up into arthroconidia; however, they are much narrower in dimension and do not share the alternate staining characteristics.
Microbiology
The hyaline saprobic fungus that has microscopic features similar to the mold form of Coccidioides immitis is:

A) Trichosporon species
B) Geotrichum species
C) Microsporum canis
D) Malbranchia species


2. Overcentrifugation may cause either a false negative result (if too much agitation is required for resuspension), or a false positive, (if centrifuged clumps cannot be completely dispersed). High concentration of IgG paraprotein, and failure to adequately wash cells can leave unbound IgG which will neutralize antiglobulin reagent. Delay of addition of antiglobulin reagent may allow previously bound IgG antibody to dissociate from red cells.
Blood bank
Which of the following might cause a false positive indirect antiglobulin test:

A) Overcentrifugation
B) High concentration of IgG paraprotein in a patient's serum
C) Failure to adequately wash cells
D) Delay of addition of antiglobulin reagent.


3. Waived tests are those considered to have an insignificant risk of erroneous results. Which of the following is NOT an example of a waived test?

A) fructosamine
B) spun hematocrit
C) blood glucose by home meters
D) potassium


4. Which of the following is NOT part of the magnification system of the microscope?

A) 10 X objective lens
B) ocular lenses-arm
C) arm
D) 4 X objective lens


5. Measuring the turnaround times of troponin tests from the emergency department would provide valuable information. This data represents circumstances in which patient safety may be compromised if results are delayed and treatment is not started as soon as possible.
A laboratory manager wants to evaluate the timeliness of patient services in order to prevent medical errors caused by delay in treatment. Measuring the turnaround time for which of these analytes would provide the most valuable information?

A) Routine glucose tests from patients on medical floors
B) Lipid panels from the outpatient clinic
C) Troponin tests from the emergency department


Solutions:

Question # 1
Answer: D
Question # 2
Answer: A
Question # 3
Answer: D
Question # 4
Answer: C
Question # 5
Answer: C

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